capralogics

Anti-TRAIL-R2 (DR5)

Background: Historically, TRAIL-R2 has been referred to as tumor necrosis factor receptor superfamily member 10B precursor, death receptor 5, TNF-related apoptosis-inducing ligand receptor 2, TRAIL receptor 2, and CD262 antigen. TRAIL induces apoptosis in various types of malignant cells without affecting normal cells.  TRAIL binds to receptors TRAIL-R1, TRAIL-R2, TRAIL-R3, and TRAIL-R4.  Upon binding TRAIL the death receptors, TRAIL-R1 (DR4) and TRAIL-R2 (DR5), signal apoptosis. 

Specificity: This antibody recognizes human and mouse TRAIL-R2 and detects a band of ~52kDa by Western blot in HL-60 and Jurkat cells and also recognizes a ~100 kD protein that may be a dimer.

Formulation: Liquid 200 µg of affinity purified antibody in 10 mM KHPO4, 140 mM NaCl with 0.1 % sodium azide

Quality Control: Western blot and/or Immunocytochemistry on cell lines HEK 293 or Jurkat

Storage: Freeze at -20 °C or colder. Stable for at least 3 years when stored at -80°C. Avoid freeze/thaw cycles.

CIO110P - Blocking Peptide TRAIL-R2 antibody $95 USD/100 µg

capralogics anti-TRAIL-R2 DR5

Anti-TRAIL-R2 IH of paraffin section human spleen ENLARGE

Contact Capralogics antibodyinfo@capralogics.com
FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number CIO110
Amount 200 µg
Price $295
Host Goat housed at Capralogics
Antigen Synthetic peptide 68APQQKRSSPSEGLCPPGHHISEDGRDC94 of the extracellular domain of human TRAIL-R2 (DR5)
Purification Peptide affinity purified
Species Cross- Reactivity Mouse
Buffer 10 mM KHPO4, 140 mM NaCl
pH 7.2
Applications and Protocols  
ELISA (peptide) 1:150,000
Western Blot (WB) 1:1000
Immunohistochemistry (IC) 1:300
Immunohistochemistry (IH) paraffin sections 1:300
Flow Cytometry (FC) 1ul/10^6 cells/100ul

 

For Research Purposes Only
Citations and Applications
Sanlioglu AD, Korcum AF, Pestereli E, Erdogan G, Karaveli S, Savas B, Griffith TS, Sanlioglu S.  TRAIL death receptor-4 expression positively correlates with the tumor grade in breast cancer patients with invasive ductal carcinoma.  Int J Radiat Oncol Biol Phys. 2007 Nov 1;69(3):716-23. Abstract IH
Aydin C, Sanlioglu AD, Karacay B, Ozbilim G, Dertsiz L, Ozbudak O, Akdis CA, Sanlioglu S.  Decoy receptor-2 small interfering RNA (siRNA) strategy employing three different siRNA constructs in combination defeats adenovirus-transferred tumor necrosis factor-related apoptosis-inducing ligand resistance in lung cancer cells.  Hum Gene Ther. 2007 Jan;18(1):39-50  Abstract IH
Hoffmann O, Priller J, Prozorovski T, Schulze-Topphoff U, Baeva N, Lunemann JD, Aktas O, Mahrhofer C, Stricker S, Zipp F, Weber JR. TRAIL limits excessive host immune responses in bacterial meningitis. J Clin Invest. 2007 Jul;117(7):2004-13.  Full Text FC 1:200 dilution
Ganten TM, K Hoffmann O, Priller J, Prozorovski T, Schulze-Topphoff U, Baeva N, Lunemann JD, Aktas O, Mahrhofer C, Stricker S, Zipp F, Weber JR. TRAIL limits excessive host immune responses in bacterial meningitis.  J Clin Invest. 2007 Jul;117(7):2004-13.   Full Text FC
Ganten TM, Koschny R, Haas TL, Sykora J, Li-Weber M, Herzer K, Walczak H.  Proteasome inhibition sensitizes hepatocellular carcinoma cells, but not human hepatocytes, to TRAIL.  Hepatology. 2005 Sep;42(3):588-97. WB   Abstract
Shah K, Bureau E, Kim DE, Yang K, Tang Y, Weissleder R, Breakefield XO.  Glioma therapy and real-time imaging of neural precursor cell migration and tumor regression.  Ann Neurol. 2005 Jan;57(1):34-41.  WB Full Text
Ganten TM, Haas TL, Sykora J, Stahl H, Sprick MR, Fas SC, Krueger A, Weigand MA, Grosse-Wilde A, Stremmel W, Krammer PH, Walczak H. Enhanced caspase-8 recruitment to and activation at the DISC is critical for sensitisation of human hepatocellular carcinoma cells to TRAIL-induced apoptosis by chemotherapeutic drugs. Cell Death Differ. 2004 Jul;11 Suppl 1:S86-96.  WB Full Text
Jin TG, Kurakin A, Benhaga N, Abe K, Mohseni M, Sandra F, Song K, Kay BK, Khosravi-Far R.  Fas-associated protein with death domain (FADD)-independent recruitment of c-FLIPL to death receptor 5.  J  Biol Chem.  2004 Dec 31;279(53):55594-601. WB Full Text
LaVallee TM, Zhan XH, Johnson MS, Herbstritt CJ, Swartz G, Williams MS, Hembrough WA, Green SJ, Pribluda VS.2-methoxyestradiol up-regulates death receptor 5 and induces apoptosis through activation of the extrinsic pathway.  Cancer Res. 2003 Jan 15;63(2):468-75.  IH Full Text
Higuchi H, Yoon JH, Grambihler A, Werneburg N, Bronk SF, Gores GJ. Bile acids stimulate cFLIP phosphorylation enhancing TRAIL-mediated apoptosis.  J Biol Chem. 2003 Jan 3;278(1):454-61. IC, WB  Full Text
Higuchi H, Bronk SF, Takikawa Y, Werneburg N, Takimoto R, El-Deiry W, Gores GJ.  The bile acid glycochenodeoxycholate induces trail-receptor 2/DR5 expression and apoptosis.  J Biol Chem. 2001 Oct 19;276(42):38610-8. IC, WB  Full Text

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