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Goat Anti-Human CCR2

Chemokine receptors are G protein-coupled receptors (GPCRs) that play a key role in inflammation and are characterized by similar sequences with a 4 cysteine motif.  The C-C family has the first 2 cysteines linked together while the C-x-C family has an amino acid in between the first 2 cysteines.  Historically, chemokine receptor type 2  has been designated C-C CKR-2, CC-CKR-2, CCR-2, Monocyte chemoattractant protein 1 receptor, MCP-1-R, and CD192 antigen.

Specificity: Peptide sequence is < 50 % identical to other chemokine receptors in this region. Cross-reactivity to mouse CCR2 not tested.

Formulation: Liquid. 200 µg of peptide affinity purified IgG antibody in 10 mM KHPO4, 140 mM NaCl with 1 mg/ml BSA and 0.1 % sodium azide.

Quality Control: IH on human spleen

Storage: Freeze at -20° C. For long term storage freeze at -70° C. Avoid freeze-thaw cycles.

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Above: IH on paraffin section of human spleen
Contact Capralogics
antibodyinfo@capralogics.com
FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number CIO118
Price $ 299
Amount 200 µg
Host Goat housed at Capralogics
Antigen

Synthetic peptide C-G17EEVTTFFDYDYGAPCHKFDVKQ39 corresponding to the N-terminal extracellular domain of human CCR2
Purification Peptide affinity purified
Species Cross- Reactivity Not tested
Buffer 10 mM KHPO4, 140 mM NaCl
pH 7.2
*
Applications and Protocols  
ELISA (peptide) 1:60,000
Western Blotting (WB)

1:1,000
Immunocytochemistry (IC) 1:200
Immunohistochemistry (IH) 1:200
Flow Cytometry (FC) ND

For Research Purposes Only
Citations and Applications
He YY, Du MR, Guo PF, He XJ, Zhou WH, Zhu XY, Li DJ.  Regulation of C-C motif chemokine ligand 2 and its receptor in human decidual stromal cells by pregnancy-associated hormones in early gestation. Hum Reprod. 2007 Oct;22(10):2733-42.  Abstract IH
Ebihara N, Yamagami S, Yokoo S, Amano S, Murakami A. Involvement of C-C chemokine ligand 2-CCR2 interaction in monocyte-lineage cell recruitment of normal human corneal stroma.  J Immunol. 2007 Mar 1;178(5):3288-92.  Abstract  IC     
Sakamoto T, Ishibashi T, Sakamoto N, Sugimoto K, Egashira K, Ohkawara H, Nagata K, Yokoyama K, Kamioka M, Ichiki T, Sugimoto N, Kurabayashi M, Suzuki K, Takuwa Y, Maruyama Y.  Endogenous NO blockade enhances tissue factor expression via increased Ca2+ influx through MCP-1 in endothelial cells by monocyte adhesion.  Arterioscler Thromb Vasc Biol. 2005 Sep;25(9): 2005-11. Full Text WB
Vestergaard C, Just H, Baumgartner Nielsen J, Thestrup-Pedersen K, Deleuran M.Expression of CCR2 on monocytes and macrophages in chronically inflamed skin in atopic dermatitis and psoriasis.  Acta Derm Venereol. 2004; 84(5):353- 8.  Abstract IH

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