capralogics

Goat Anti-Human CCR6 External Cellular Domain

Chemokine receptors are G protein-coupled receptors (GPCRs) and play a key role in inflammation and are characterized by similar sequences with a 4 cysteine motif.  The C-C family has the first 2 cysteines linked together while the C-x-C family and an amino acid in between the first 2 cysteines.  Historically, chemokine receptor C-C chemokine receptor type 6 (CCR6) has been designated as C-C CKR-6, CC-CKR-6, CCR-6, LARC receptor, GPR-CY4, GPRCY4, Chemokine receptor-like 3 (CKR-L3), DRY6, G-protein coupled receptor 29, and CD196 antigen.

Specificity: This antibody recognizes human CCR6 and the peptide sequence is < 50 % identical to other human chemokine receptors in this region.

Formulation: Liquid, 200 µg of affinity purified antibody in 10 mM KHPO4, 140 mM NaCl with 1 mg/ml BSA and 0.1 % sodium azide

Quality Control: Immunohistochemistry on human spleen

Storage: Freeze at -20° C. For long term storage freeze at -70° C. Avoid freeze-thaw cycles.

capralogics anti CCR6

Above: IH on paraffin section of human spleen

For Research Purposes Only
Contact Capralogics
antibodyinfo@capralogics.com
FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number CIO129
Amount 200 µg
Price $ 299 buy online
Host Goat housed at Capralogics
Antigen

Synthetic peptide YFVSVNTSYYSVDSEMLLCSLQEVRQFSR to amino acid residues 18 - 46 of human CCR6

Purification Peptide affinity purified
Cross- Reactivity Mouse immunohistochemistry where linear epitopes are accessible
Buffer 10 mM KHPO4, 140 mM NaCl
pH 7.2
*
Applications and Protocols  
ELISA (peptide) 1:35,000
Western Blotting (WB)

1:250
Immunohistochemistry (IH) 1:125
Flow Cytometry (FC) 2-4ul/10^6 cells/50-100 ul

capralogics anti CCR6

Above: IH on paraffin section of mouse spleen
Citations and Applications
Veerayutthwilai O, Byers MR, Pham T-TT, Darveau RP, Dale BA. Differential regulation of immune responses by odontoblasts. Oral Microbiol Immunol 2007: 22: 5–13  Abstract IH
Rubie C, Frick VO, Wagner M, Weber C, Kruse B, Kempf K, Konig J, Rau B, Schilling M.Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases.  World J Gastroenterol 2006 12(41):6627-6633 Full Text WB
Rubie C, Frick VO, Wagner M, Rau B, Weber C, Kruse B, Kempf K, Tilton B, Konig J, Schilling M.  Enhanced expression and clinical significance of CC-chemokine MIP-3 alpha in hepatocellular carcinoma.  Scand J Immunol. 2006 Jun;63(6):468-77.  Abstract IH, WB
Rubie C, Oliveira V, Kempf K, Wagner M, Tilton B, Rau B, Kruse B, Konig J, Schilling M.  Involvement of chemokine receptor CCR6 in colorectal cancer metastasis.  Tumour Biol. 2006 27(3):166-74.  Abstract  IH, WB

Capralogics Home>> Chemokine Receptor Index