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Goat Anti-Human ß Chemokine Receptor D6

D6 is expressed on the lymphatic endothelium and leukocytes.  Defined as a "silent" chemokine receptor, D6 has no G coupling protein motifs.  No signalling occurs upon chemokine binding and D6 functions as a scavenger receptor internalizing and degrading chemokines leading to a dampening of inflammatory processes.


Specificity: Peptide sequence is < 50 % identical to other chemokine receptors in this region and cross-reactivity to mouse D6 was not tested.

Formulation: CI0130 is liquid 200 µg of affinity purified antibody in 10 mM KHPO4, 140 mM NaCl with 1 mg/ml BSA and 0.1 % sodium azide. CI0171 is liquid 100 µg of biotin labeled affinity purified antibody in 10 mM KHPO4, 140 mM NaCl with 1 mg/ml BSA and 0.1 % sodium azide.

Quality Control: Immunohistochemistry on human spleen

Storage: Freeze at -20° C. For long term storage freeze at -70° C. Avoid freeze-thaw cycles.

capralogics

IH on paraffin section of human spleen

CIO130P - Blocking Peptide to Antibody $95 USD per 100 µg

Contact Capralogics
antibodyinfo@capralogics.com
FAX 1-413-643-0067
Phone 1-800-975-6866

CIO130 unconjugated CIO171 biotin conjugate

Amount 200 µg or 100 µg biotinylated
Price $ 299 buy online
Host Goat housed at Capralogics
Antigen

Synthetic peptide C-L10ATEDADSENSSFYYYDYLDEVAFM35L corresponding to the N-terminal extracellular domain of human D6

Purification Peptide affinity purified
Species Cross- Reactivity Not tested
Buffer 10 mM KHPO4, 140 mM NaCl
pH 7.2
*
Applications and Protocols  
ELISA (peptide) 1:50,000
Western Blotting (WB)

1:500

Immunohistochemistry (IH) 1:125
Flow Cytometry (FC) 2-4ul/10^6 cells/50-100 ul

For Research Purposes Only

Citation and Application
Neil SJ, Aasa-Chapman MM, Clapham PR, Nibbs RJ, McKnight A, Weiss RA.  The promiscuous CC chemokine receptor D6 is a functional coreceptor for primary isolates of human immunodeficiency virus type 1 (HIV-1) and HIV-2 on astrocytes.  J Virol.  2005 Aug;79(15):9618-24.  Full Text FC

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