Human pp36 T-cell Adaptor Protein - LAT

Background :  Transmembrane adaptor molecules are an important category of lymphoid cell markers.  The linker for activation of T cells (LAT) has historyicall been designated as 36 kDa phospho-tyrosine adapter protein, pp36, and p36-38.  LAT plays a role with after antigen binding to TCR in initiatiating of T-cell signaling cascades leading to IL-2 production. Disruption of LAT prevents early thymocyte maturation.  LAT is important for NK signaling and platelet activation.

Specificity: This antibody binds to a ~40 kD protein in Western blots.

Formulation: Liquid 200 µg of peptide affinity purified IgG antibody in 10 mM KHPO4, 140 mM NaCl with 1 mg/ml BSA and 0.1 % sodium azide.

Quality Control: Western Blotting on mouse lung or human T cell line Jurkat and K562 cells

Storage: Freeze at -20° C. For long term storage freeze at -70° C. Avoid freeze-thaw cycles.

 

For Research Purposes Only

 

 

Product Number CIO138
Price $ 195
Amount 200 µg
Host Rabbit
Antigen

Synthetic peptide CHRLPGSYDSTSSDSLYPRGI corresponding to amino acids 29-49 at N-terminus of human pp36, a tyrosine-phosporylated adaptor protein.
Purification Peptide affinity purified
Species Cross- Reactivity Mouse
Buffer 10 mM KHPO4, 140 mM NaCl
pH 7.2
*
Applications  
ELISA (peptide) 1:100,000
Western Blotting

1:1,000
Immunohistochemistry 1:300

References:

Lindquist JA, Simeoni L, Schraven B.  Transmembrane adapters: attractants for cytoplasmic effectors.  Immunol Rev. 2003 Feb;191:165- 82.  Abstract

Weber JR et al 1998 Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T Cells and natural killer cells. J Exp Med 1998, 187, 1157-61. Full Text

Capralogics >> Signaling Intermediates