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Antibody to Human IL-21 N-terminus


DATASHEET


Specificity: Peptide sequence is < 50 % identical to other interleukins in this region. The antibody recognizes human IL-21. Not tested for cross-reactivity to mouse IL-21.

Formulation: Liquid 100 µg of affinity purified antibody in 10 mM KHPO4, 140 mM NaCl with 0.1 % sodium azide.

Quality Control: Immunocytochemistry on human spleen and/or human tonsil

Storage: Freeze at -20° C. For long term storage freeze at -70° C. Avoid freeze-thaw cycles.

capralogics anti-IL21

Above: Immunocytochemistry of human tonsil with goat antibody to N-terminus of human IL-21

For Research Purposes Only

Contact Capralogics
antibodyinfo@capralogics.com FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number CIO140

Price $ 295
Amount 100 µg
Host Goat
Antigen Synthetic peptide C-40RQLIDIVDQLKNYVNDLVPEFLPAPEDVE68 corresponding to N-terminus region of human IL-21
Sequence Alignment

Human IL-21
RQLIDIVDQLKNYVNDLVPEFLPAPEDVE RHLIDIVEQLK I YENDLDPELLSAPQDVK

Mouse IL-21

Antibody Concentration

1.0 mg/mL

Purification Peptide affinity purified
Species Cross- Reactivity not tested
Buffer 10 mM KHPO4, 140 mM NaCl
pH 7.2

Applications and Protocols

Can be used for ELISA for native human IL-21 in combination with Product CI0139 with the Suggested Protocol.

Note: Some commercially available lots of rhIL-21 do not work for an ELISA standard curve due possibly to folding differences from disulfide bonds.

ELISA (for Peptide) 1:100000
Immunocytochemistry See Protocol 1:500
Flow Cytometry ND
References  
1.
Kim HR, Hong MS, Dan JM, Kang I.  Altered IL-7Ralpha expression with aging and the potential implications of IL-7 therapy on CD8+ T-cell immune responses.  Blood. 2006 Apr 1;107(7):2855-62.  Full Text
2.
Parrish-Novak J et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function. Nature 2000, 408 (6808), 57-63 Abstract

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