capralogics

Antibody to Mouse CCR2

DATASHEET

Chemokine receptors are G protein-coupled receptors (GPCRs) that play a key role in inflammation and are characterized by similar sequences with a 4 cysteine motif.  The C-C family has the first 2 cysteines linked together while the C-x-C family has an amino acid in between the first 2 cysteines.  Historically, murine chemokine receptor type 2  has been designated C-C CKR-2, CC-CKR-2, CCR-2, JE/FIC receptor, MCP-1 receptor, and CD192 antigen.

Specificity: This antibody recognizes mouse CCR2 and the peptide sequence is < 50 % identical to other chemokine receptors in this region.

Formulation: Liquid 200 µg of affinity purified antibody in 10 mM KHPO4, 140 mM NaCl with 0.1 % sodium azide and 1 mg/ml BSA

Quality Control: Immunohistochemistry on paraffin sections of mouse spleen

Storage: Freeze at -20 °C or colder. Stable for at least 3 years when stored at -80°C. Avoid freeze/thaw cycles.

capralogics anti CCR2 mouse

IH of CI0153 antibody to CCR2 on paraffin section mouse spleen
Contact Capralogics
antibodyinfo@capralogics.com
FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number CIO153
Price $ 299 buy online
Amount 200 µg in 200 µl
Host Species Goat housed at Capralogics
Antigen Synthetic peptide SHSLFTRSIQELDEGATTPYDYDDGEPC corresponding to aa 18-45 of the N-terminal domain of mouse CCR2
Antibody Concentration:

1.0 mg/mL
Purification Peptide Affinity purified
Species Cross- Reactivity not tested
Buffer 10 mM KHPO4, 140 mM NaCl
pH 7.2
*
Applications and Protocols  
Western Blot 1:500
Immunocytochemistry (IC) (acetone fixed cells) 1:200
Immunohistochemistry (IH) (paraffin sections) 1:125
Flow Cytometry (FC) 1:20

 

For Research Purposes Only
Citations and Applications
El Annan J, Chauhan SK, Ecoiffier T, Zhang Q, Saban DR, D. Characterization of effector T cells in dry eye disease. Invest Ophthalmol Vis Sci. 2009 Aug;50(8):3802-7 Abstract FC
Ishida Y, Gao JL, Murphy PM.   Chemokine receptor CX3CR1 mediates skin wound healing by promoting macrophage and fibroblast accumulation and function.  J Immunol. 2008 Jan 1;180(1):569-79. Full Text IH
El-Hage N, Wu G, Ambati J, Bruce-Keller AJ, Knapp PE, Hauser KF.  CCR2 mediates increases in glial activation caused by exposure to HIV-1 Tat and opiates. J Neuroimmunol. 2006 Sep;178(1-2):9-16.  Abstract IC

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