Antibody to Mouse CCR3

DATASHEET

Chemokine receptors are G protein-coupled receptors (GPCRs) that play a key role in inflammation and are characterized by similar sequences with a 4 cysteine motif. The C-C family has the first 2 cysteines linked together while the C-x-C family has an amino acid in between the first 2 cysteines. Historically, murine chemokine receptor type 3 (CCR3) has been designated as C-C CKR-3, CC-CKR-3, CCR-3, CKR3, Macrophage inflammatory protein 1-alpha receptor-like 2, MIP-1 alpha RL2, and CD193 antigen.

Specificity: This antibody recognizes mouse CCR3 and the peptide sequence is < 50 % identical to other chemokine receptors in this region.

Formulation: Liquid 200 µg of affinity purified antibody in 10 mM KHPO4, 140 mM NaCl with 0.1 % sodium azide and 1 mg/ml BSA

Quality Control: Immunohistochemistry on paraffin sections of mouse spleen

Storage: Freeze at -20 °C or colder. Stable for at least 3 years when stored at -80°C. Avoid freeze/thaw cycles.

capralogics

Above: Immunohistochemistry of CI0154 antibody to mouse CCR3 on paraffin section of mouse spleen
Contact Capralogics
antibodyinfo@capralogics.com
FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number CIO154
Price $ 299
Amount 200 µg in 100 µl
Host Species Goat housed at Capralogics
Antigen Synthetic peptide TDEIKTVVESFETTPYEYEWAPPC corresponding to aa 5-28 of the N-terminal domain of mouse CCR3
Antibody Concentration

2.0 mg/mL
Purification Peptide affinity purified
Species Cross- Reactivity not tested
Buffer 10 mM KHPO4, 140 mM NaCl
pH 7.2
*
Applications and Protocols  
Western Blot 1:1000
Immunocytochemistry (acetone fixed cells) 1:400
Immunohistochemistry (paraffin sections) 1:250
Flow Cytometry ND

For Research Purposes Only
Reference
Gao JL, Murphy PM.  Cloning and differential tissue-specific expression of three mouse beta chemokine receptor-like genes, including the gene for a functional macrophage inflammatory protein-1 alpha receptor.  J Biol Chem.  1995 Jul 21;270(29):17494-501  Full Text

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