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anti-human Adiponectin Receptor AdipoR2 N-terminal domain

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Description: Adiponectin receptor 2 (AdipoR2) is an integral membrane protein with seven transmembrane domains with an internal N-terminus and an external C-terminus. AdipoR2 is highly conserved among species and expressed mainly in the liver and skeletal muscle. Expression of AdipoR2 in the brain correlates with brain development and size in mice.

Specificity: Peptide sequence is < 50 % identical to other receptors in this region. The antibody recognizes AdipoR2 in human tissue.

Sequence Alignment:

Human AdipoR2
CSRTPEPDIRLRKGHQLDGTRRGDNDSHQG
CTRTPEPDIRLRKGHQLDDTRGGNNDNHHG
Rat AdipoR2

Human AdipoR2
CSRTPEPDIRLRKGHQLDGTRRGDNDSHQG
CTRTPEPDIRLRKGHQLDDTRGSNNDNYQG
Mouse AdipoR2

Formulation: Liquid 200 µg of affinity purified antibody at 1 mg/mL in 10 mM KHPO4, 140 mM NaCl with 1 mg/mL BSA and 0.1 % sodium azide.

Quality Control: IH on paraffin sections of human liver and muscle

Storage: Freeze at -20° C. For long term storage freeze at -80° C. Avoid freeze-thaw cycles.

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IH paraffin section human liver with anti-Adipo R2 N-terminal region antibody Larger Image
Contact Capralogics
antibodyinfo@capralogics.com
FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number CIO165
Price $ 295 buy online
Amount 200 µg in 200 µl
Host Goat housed at Capralogics
Antigen

30 amino acid (aa) synthetic peptide CSRTPEPDIRLRKGHQLDGTRRGDNDSHQG corresponding to aa 11-40 of the N-terminal domain of human AdipoR2
Purification Peptide affinity purified
Species Cross- Reactivity mice, rat possibly
Buffer 10 mM KHPO4, 140 mM NaCl
pH 7.2
Applications and Protocols  
Western Blotting (WB) 1:1000
Immunocytochemistry (IC) acetone fixed cells

1:400
Immunohistochemistry (IH) paraffin sections 1:250
Flow Cytometry ND

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IH paraffin section human muscle with anti-Adipo R2 N-terminal region antibody

For Research Purposes Only
References
Bjursell M, Ahnmark A, Bohlooly-Y M, William-Olsson L, Rhedin M, Peng XR, Ploj K, Gerdin AK, Arnerup G, Elmgren A, Berg AL, Oscarsson J, Lindén D. Opposing effects of adiponectin receptors 1 and 2 on energy metabolism.  Diabetes. 2007 Mar;56(3):583-93.  Full Text
Kadowaki T, Yamauchi T, Kubota N, Hara K, Ueki K, Tobe K. Adiponectin and adiponectin receptors in insulin resistance, diabetes, and the metabolic syndrome.  J Clin Invest. 2006 Jul;116(7):1784-92.  Full Text
Rasmussen MS, Lihn AS, Pedersen SB, Bruun JM, Rasmussen M, Richelsen B.  Adiponectin receptors in human adipose tissue: effects of obesity, weight loss, and fat depots.  Obesity (Silver Spring). 2006 Jan;14(1):28-35. Full Text
Staiger H, Kaltenbach S, Staiger K, Stefan N, Fritsche A, Guirguis A, Peterfi C, Weisser M, Machicao F, Stumvoll M, Haring HU.   Expression of adiponectin receptor mRNA in human skeletal muscle cells is related to in vivo parameters of glucose and lipid metabolism.  Diabetes. 2004 Sep;53(9):2195-201.  Full Text
Yamauchi T, et al. Cloning of adiponectin receptors that mediate antidiabetic metabolic effects.  Nature. 2003 Jun 12;423(6941):762-9.  Abstract

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