rabbit anti-FGF2

	Rabbit anti-FGF2
Background: Fibroblast growth factor 2 (FGF2, FGF-2), also known as basic fibroblast growth factor (bFGF, b-FGF), is mitogenic and angiogenic. FGF2 plays a key role in wound healing, development of limb and nervous systems, and tumorogenesis. Historically, this 288 amino acid fibroblast growth factor family (FGF) member was known as heparin-binding growth factor 2 precusor and prostatropin. Specificity: Rabbits were immunized with human recombinant FGF2. Anti-FGF2 is able to neutralize in growth assays. Purification and Formulation: Serum immunoglobulin was precipitated twice by ammonium sulfate fractionation and then dissolved and dialyzed against phosphate buffered saline (PBS) containing 0.015 M sodium phosphate, pH 7.5, 0.15 M NaC1, with sodium azide 0.02% as a preservative. Storage: Short term storage at 4° C. Freeze at -20° C or colder for long term storage. Avoid freeze-thaw cycles. Contact Capralogics antibodyinfo@capralogics.com FAX 1-413-643-0067 Phone 1-800-975-6866	Product Code	P00853
	Price	$ 295
	Amount	150 ug
	Host	Rabbit
	Antigen	FGF2
	Concentration	1 mg/mL
	Purification	Ammonium sulfate fractionation
	Buffer	PBS pH 7.5
	Applications: For protocols and images see citations
	Growth Neutralization (GN): 10 ug/mL
	Immunofluorescence (IF): 1:100
	Immunohistochemistry (IH): 1:100
	Immunoprecipitation (IP): 2 -4X molar excess
	Immunoblotting (IB): 1:500
	ECL Western Blot (WB): 1:500
	For Research Purposes Only

Citations and Applications

Papetti M, Shujath J, Riley KN, Herman IM. FGF-2 antagonizes the TGF-beta1-mediated induction of pericyte alpha-smooth muscle actin expression: a role for myf-5 and Smad-mediated signaling pathways. Invest Ophthalmol Vis Sci. 2003 Nov;44(11):4994-5005. Full Text GN, IF, IP

Cox CM, Poole TJ. Angioblast differentiation is influenced by the local environment: FGF-2 induces angioblasts and patterns vessel formation in the quail embryo. Dev Dyn. 2000 Jun;218(2):371-82. Abstract GN

Cao J, Mathews MK, McLeod DS, Merges C, Hjelmeland LM, Lutty GA. Angiogenic factors in human proliferative sickle cell retinopathy. Br J Ophthalmol. 1999 Jul;83(7):838-46. Full Text IH

Acevedo AD, Bowser SS, Gerritsen ME, Bizios R. Morphological and proliferative responses of endothelial cells to hydrostatic pressure: role of fibroblast growth factor. J Cell Physiol. 1993 Dec;157(3):603-14. Abstract IC

Carlone DL, Rider V. Embryonic modulation of basic fibroblast growth factor in the rat uterus. Biol Reprod. 1993 Oct;49(4):653-65. Full Text IH, WB

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