Antibody to PIP5KIgamma

Datasheet

Background: This antibody binds to PIP5KIgamma, also referred to Phosphatidylinositol-4-phosphate 5-kinase type-1 gamma, Phosphatidylinositol-4-phosphate 5-kinase type I gamma, PtdIns(4)P-5-kinase gamma, and PtdInsPKIgamma. The PIP kinases are classifed with regards to signaling specificity as  type I, II, and III.  The type I and II PIP kinases include alpha, beta, and gamma isoforms and several splice variants. 

Specificity: Binds to a 85-90 Kd protein as shown by Western Blotting. Binding can be blocked by the peptide immunogen which is available for purchase.

Formulation: 200 ug per vial in KHPO4 buffer with 1 mg/ml BSA and 0.1% sodium azide

Quality Control: Western Blot on mouse spleen and brain lysates

Storage: Freeze at -20° C. For long term storage freeze at -70° C. Avoid freeze-thaw cycles.

For Research Purposes Only
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Phone 1-800-975-6866

Product Number CIO108
Price buy online
Host Rabbit
Antigen

synthetic peptide with added cysteine C-622ASDEEDAPSTDIYFPTDERS641 to C-terminal region of PIP5KIgamma

Purification Peptide affinity column
Species Cross-Reactivity Human
Buffer

IgG in KHPO4 buffer

Applications and Protocols

 
Immunocytochemistry 1:150
Western Blot 1:500
ELISA (peptide) 1:25,000

CIO108P - Blocking Peptide to Antibody per 100 µg

References

NCBI: O70161. Reports Phosphatidylinosi...[gi:78099089]

Hisamitsu Ishihara et. al. (1998) Type 1 phosphatidylinositol-4-phosphate 5-kinases. J Biol. Chem. 273 (15) 8741-8748 Full Text