Anti-Mouse CCR4

Goat Anti-Mouse CCR4 (N-Terminal region)

Chemokine receptors are G protein-coupled receptors (GPCRs) and play a key role in inflammation and are characterized by similar sequences with a 4 cysteine motif. The C-C family has the first 2 cysteines linked together while the C-x-C family and an amino acid in between the first 2 cysteines. Historically, murine chemokine receptor type 4 (CCR4) has been designated as C-C CKR-4, CC-CKR-4, and CCR-4.

Specificity: Peptide sequence is < 50 % identical to other human chemokine receptors in this region. The antibody recognizes mouse CCR4 and has no cross-reactivity to human CCR4.

Formulation: Peptide affinity purified IgG in 10 mM KHPO4, 140 mM NaCl with 1 mg/ml BSA and 0.1% sodium azide

Quality Control: Immunohistochemistry on mouse spleen

Storage: Freeze at -20° C. For long term storage freeze at -70° C. Avoid freeze-thaw cycles.

CIO122P - Blocking Peptide to Antibody per 100 µg

For Research Purposes Only

info@capralogics.com
Phone 1-800-975-6866
FAX 1-413-643-0067

Product Number CIO122

Amount	200 µg
Price	buy online
Host	Goat housed at Capralogics
Antigen	Synthetic peptide DTTQDETVYNSYYFYESMP-C corresponds to amino acid residues 8 - 26 of mouse CCR4 with cysteine added for coupling to a carrier protein
Purification	Peptide affinity purified
Species Cross-Reactivity	None to human CCR4
Buffer	10 mM KHPO4, 140 mM NaCl
pH	7.2

Applications and Protocols
ELISA (peptide)	1:25,000
Western Blotting (WB)	1:500
Immunohistochemistry (IH)	1:250
Flow Cytometry (FC)	2-4ul/10^6 cells/50-100ul

Citations and Applications

Ferguson AR, Engelhard VH. CD8 T cells activated in distinct lymphoid organs differentially express adhesion proteins and coexpress multiple chemokine receptors. J Immunol. 2010 Apr 15;184(8):4079-86. Abstract FC

Raimondi G, Sumpter TL, Matta BM, Pillai M, Corbitt N, Vodovotz Y, Wang Z, Thomson AW Mammalian target of rapamycin inhibition and alloantigen-specific regulatory T cells synergize to promote long-term graft survival in immunocompetent recipients. J Immunol. 2010 Jan 15;184(2):624-36 Abstract FC

Guo Z, Jang MH, Otani K, Bai Z, Umemoto E, Matsumoto M, Nishiyama M, Yamasaki M, Ueha S, Matsushima K, Hirata T, Miyasaka M. CD4+CD25+ regulatory T cells in the small intestinal lamina propria show an effector/memory phenotype. Int Immunol. 2008 Mar;20(3):307-15. Abstract FC

Kanagawa N, Niwa M, Hatanaka Y, Tani Y, Nakagawa S, Fujita T, Yamamoto A, Okada N. CC-chemokine ligand 17 gene therapy induces tumor regression through augmentation of tumor-infiltrating immune cells in a murine model of preexisting CT26 colon carcinoma. Int J Cancer. 2007 Nov 1;121(9):2013-22. Abstract FC

Khader SA, Bell GK, Pearl JE, Fountain JJ, Rangel-Moreno J, Cilley GE, Shen F, Eaton SM, Gaffen SL, Swain SL, Locksley RM, Haynes L, Randall TD, Cooper AM. IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge. Nat Immunol. 2007 Apr;8(4):369-77. Abstract FC

Tsunemi Y, Saeki H, Nakamura K, Nagakubo D, Nakayama T, Yoshie O, Kagami S, Shimazu K, Kadono T, Sugaya M, Komine M, Matsushima K, Tamaki K. CCL17 transgenic mice show an enhanced Th2-type response to both allergic and non-allergic stimuli. Eur J Immunol. 2006 Aug;36(8):2116-27. Abstract IH

Ochando JC, Homma C, Yang Y, Hidalgo A, Garin A, Tacke F, Angeli V, Li Y, Boros P, Ding Y, Jessberger R, Trinchieri G, Lira SA, Randolph GJ, Bromberg JS. Alloantigen-presenting plasmacytoid dendritic cells mediate tolerance to vascularized grafts. Nat Immunol. 2006 Jun;7(6):652-62. Abstract FC

Morimoto Y, Bian Y, Gao P, Yashiro-Ohtani Y, Zhou XY, Ono S, Nakahara H, Kogo M, Hamaoka T, Fujiwara H. Induction of surface CCR4 and its functionality in mouse Th2 cells is regulated differently during Th2 development. J Leukoc Biol. 2005 Sep;78(3):753-61. Full Text FC

Jakubzick C, Wen H, Matsukawa A, Keller M, Kunkel SL, Hogaboam CM. Role of CCR4 ligands, CCL17 and CCL22, during Schistosoma mansoni egg-induced pulmonary granuloma formation in mice. Am J Pathol. 2004 Oct;165(4):1211-21 Full Text IH