capralogics inc

Mouse Toll-like receptor 9 (TLR9)

Toll-like receptors (TLRs) are membrane proteins on many cells involved with innate immunity such as macrophages, dendritic cells, neutrophils, mucosal epithelial cells, and endothelial cells.  Ten different mammalian TLRs have been identified and all have distinct specificities for microbial ligands.  Upon recognition of a microbial product, TLRs initiate a signaling pathway to activate innate immunity.  TLR9 recognizes bacterial and protozoan associated CpG DNA.

Specificity: Peptide sequence is < 50 % identical to other mouse TLR receptors in this region. The antibody recognizes mouse TLR9 and human TLR9.

Sequence Alignment:
LSLKYNNLTKVPRQLPPSLEY Mouse TLR9
LSLKYNNLT+VPRQLPPSLEY
LSLKYNNLTEVPRQLPPSLEY Rat TLR9

LSLKYNNLTKVPRQLPPSLEY Mouse TLR9
LSLKYNNLT+VPR+LP+SLEY
LSLKYNNLTVVPRNLPSSLEY Human TLR9

Formulation: 100 µl liquid 200 µg of affinity purified antibody at 2 mg/mL in 10 mM KHPO4, 140 mM NaCl with 1 mg/mL BSA and 0.1 % sodium azide.

Quality Control: IH on paraffin sections of mouse spleen and thymus

Storage: Freeze at -20° C. For long term storage freeze at -80° C. Avoid freeze-thaw cycles.

TLR9 IH on paraffin section of mouse spleen

TLR9 IH on paraffin section of mouse thymus

For Research Purposes Only
Contact Capralogics
antibodyinfo@capralogics.com
FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number CIO158
Price buy online
Amount 200 µg in 200 µl
Host Goat
Antigen

Synthetic peptide LSLKYNNLTKVPRQLPPSLEY-C corresponding to amino acids 204-224 of the N-terminal domain of mouse TLR9
Purification Peptide affinity purified
Species Cross- Reactivity human
Buffer 10 mM KHPO4, 140 mM NaCl
pH 7.2
Applications and Protocols  
Western Blotting 1:500
Immunocytochemistry acetone fixed cells

1:200
Immunohistochemistry (IH) paraffin sections 1:125
Flow Cytometry ND

TLR9 IH on paraffin section of human spleen

TLR9 IH on paraffin section of human tonsil

 
Reference  
Chuang TH, Ulevitch RJ. Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9.  Eur Cytokine Netw. 2000 Sep;11(3):372-8.  Abstract

 

.