capralogics
anti-Epidermal Growth Factor Receptor (EGFr)

DATASHEET

Description: This EGFr antibody recognizes a region of the internal domain. Antibody binding blocks phosphorylation and does not interfere with EGF binding.

Product: Protein G purified immunoglobulin in phosphate buffered saline, pH 7.4. Large volumes of antisera to EGFr available for sale.

Storage: Aliquot and store at -20° C or colder. Once thawed store at 4° C with the addition of 0.1% sodium azide or other preservative. Avoid repeated freeze-thaw cycles.

 

 

 

 

For Research Purposes Only
info@capralogics.com

FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number P00367
Amount 250 µg IgG in PBS at 1.84 mg/mL
Price  
Host Sheep housed at Capralogics
Antigen

A synthetic 20 amino acid peptide of human EGFr representing a portion of the internal domain proximal to a phosphorylation region
Species cross-reactivity mouse, rat, chicken EGFr
Purification Protein G purified sheep imunoglobulin

Applications:
Western Blot (WB) Immunoblotting (IB) - Dilution range 10 - 50 µg/mL dependent on receptor concentration
Immunoprecipitation (IP) - A431 epidermoid carcinoma 10 - 12 ug/mL. Protein kinase activity is measurable on the immunoprecipitated receptor.
Immunohistochemistry - Paraffin (IH-P) 5 µg/mL
Immunocytochemistry (IC) Formalin fixed cells: 25 - 100 µg/mL dependent on receptor concentration
Citations and Applications
Ahmad Z, Brown CM, Patel AK, Ryan AF, Ongkeko R, Doherty JK. Merlin knockdown in human Schwann cells: clues to vestibular schwannoma tumorigenesis. Otol Neurotol. 2010 Apr;31(3):460-6. Abtract IB

Roepstorff K, Grandal MV, Henriksen L, Knudsen SL, Lerdrup M, Grøvdal L, Willumsen BM, van Deurs B. Differential effects of EGFR ligands on endocytic sorting of the receptor. Traffic. 2009 Aug;10(8):1115-2 Full Text WB
Pedersen NM, Breen K, Rødland MS, Haslekås C, Stang E, Madshus IH. Expression of epidermal growth factor receptor or ErbB3 facilitates geldanamycin-induced down-regulation of ErbB2. Mol Cancer Res. 2009 Feb;7(2):275-84.  Full Text  IB
Umebayashi K, Stenmark H, Yoshimori T. Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation. Mol Biol Cell. 2008 Aug;19(8):3454-62. Full Text IC, IB, IP
Bache KG, Stuffers S, Malerod L, Slagsvold T, Raiborg C, Lechardeur D, Walchli S, Lukacs GL, Brech A, Stenmark H.  The ESCRT-III subunit hVps24 is required for degradation but not silencing of the epidermal growth factor receptor.  Mol Biol Cell. 2006 Jun;17(6):2513-23.  Full Text  IB
Nahmias Y, Casali M, Barbe L, Berthiaume F, Yarmush ML.  Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary rat and human hepatocytes.  Hepatology. 2006 Feb;43(2):257-65.  Abstract IC
White IJ, Bailey LM, Aghakhani MR, Moss SE, Futter CE.  EGF stimulates annexin 1-dependent inward vesiculation in a multivesicular endosome subpopulation.  EMBO J. 2006 Jan 11;25(1):1-12. Full Text IC
Haslekas C, Breen K, Pedersen KW, Johannessen LE, Stang E, Madshus IH.  The inhibitory effect of ErbB2 on epidermal growth factor-induced formation of clathrin-coated pits correlates with retention of epidermal growth factor receptor-ErbB2 oligomeric complexes at the plasma membrane.  Mol Biol Cell. 2005 Dec;16(12):5832-42.  Full Text IC, IP
Hsu YT, Hou Q, Zhou H, Mohanty S, Litvinchuk A.  Differential immunoscreening identifies a glycosylation variant of the epidermal growth factor receptor in ME-180 cervical carcinoma cells.Hybridoma (Larchmt). 2005 Oct;24(5):225-30. Abstract WB
Longva KE, Pedersen NM, Haslekas C, Stang E, Madshus IH Herceptin-induced inhibition of ErbB2 signaling involves reduced phosphorylation of Akt but not endocytic down-regulation of ErbB2.  Int J Cancer. 2005 Sep 1;116(3):359-67. Abstract IB

Grovdal LM, Stang E, Sorkin A, Madshus IH.  Direct interaction of Cbl with pTyr 1045 of the EGF receptor (EGFR) is required to sort the EGFR to lysosomes for degradation. Exp Cell Res. 2004 Nov 1;300(2):388-95.  Abstract IC
Enwere E, Shingo T, Gregg C, Fujikawa H, Ohta S, Weiss S.  Aging results in reduced epidermal growth factor receptor signaling, diminished olfactory neurogenesis, and deficits in fine olfactory discrimination.  J Neurosci. 2004 Sep 22;24(38):8354-65. Full Text IH, WB
Oksvold MP, Thien CB, Widerberg J, Chantry A, Huitfeldt HS, Langdon WY.  UV-radiation-induced internalization of the epidermal growth factor receptor requires distinct serine and tyrosine residues in the cytoplasmic carboxy-terminal domain.  Radiat Res. 2004 Jun;161(6):685-91. Abstract IC, WB
Oksvold MP, Thien CB, Widerberg J, Chantry A, Huitfeldt HS, Langdon WY.  Serine mutations that abrogate ligand-induced ubiquitination and internalization of the EGF receptor do not affect c-Cbl association with the receptor.  Oncogene. 2003 Nov 20;22(52):8509-18. Abstract IC, WB
Buteau J, Foisy S, Joly E, Prentki M.  Glucagon-like peptide 1 induces pancreatic beta-cell proliferation via transactivation of the epidermal growth factor receptor. Diabetes. 2003 Jan;52(1):124-32.  Full Text IP, WB
Denning MF, Dlugosz AA, Cheng C, Dempsey PJ, Coffey RJ Jr, Threadgill DW, Magnuson T, Yuspa SH.  Cross-talk between epidermal growth factor receptor and protein kinase C during calcium-induced differentiation of keratinocytes.  Exp Dermatol. 2000 Jun;9(3):192-9.  Abstract IB
Galcheva-Gargova Z, Theroux SJ, Davis RJ.  The epidermal growth factor receptor is covalently linked to ubiquitin.  Oncogene.  1995 Dec 21;11(12):2649-55.  Abstract
Meisner H, Daga A, Buxton J, Fernandez B, Chawla A, Banerjee U, Czech MP.  Interactions of Drosophila Cbl with epidermal growth factor receptors and role of Cbl in R7 photoreceptor cell development. Mol Cell Biol.  1997 Apr;17(4):2217-25.  Full Text IC, IP, WB