capralogics
Sheep anti-Erythropoietin Receptor (EpoR)

DATASHEET


Description: The human erythropoietin receptor (EpoR) is located on stem and mature cells of the hematopoietic system. When activated by its native ligand, erythropoietin (Epo), EpoR mediates proliferation and maturation of the erythroid precursor cells leading to expansion of red cell populations. Recombinant Epo is currently used for treatment of patients undergoing erythrocyte depletion due to dialysis for kidney failure.

Antibody Description: The immunogen used was the cDNA of the extracellular domain of human EpoR expressed as a glutathione-S-transferase (GST) fusion protein in E. coli. Antibody titers and specificity were analyzed by ELISA assay using control samples by varying concentration of GST-EpoR and control anti-GST antibody. Affinity purified antibody was found to detect EpoR at concentrations of 50 ng/ml. Neutralization of ligand binding has been detected using erythroleukemia cell line TF-1 with 3000 Epo receptors/cell to block Iodine 125 labeled erythropoietin ligand binding. This antibody will recognize EpoR-GST at its migration of 49 kD on SDS-PAGE. High quality secondary anti-sheep reagents are essential for this assay. Large volumes of antisera to EpoR are available for sale.

Storage: Aliquot and store at -20° C or colder, once thawed store at 4° C with the addition of 0.1% sodium azide. Avoid repeated freeze-thaw cycles.

For Research Purposes Only

info@capralogics.com

FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number P00368

Price   buy online
Amount 250 µg in 0.15 M PBS pH 7.4, 1.32 mg/mL
Host Sheep housed at Capralogics
Antigen

Partial cDNA of the extracellular domain of human EpoR expressed as a GST fusion protein in E. Coli

Species cross-reactivity mouse
Purification Protein G purified sheep IgG
Applications  
Immunoprecipitation (IP) 10 µg/mL
Western Blot (WB) 10 µg/mL
Neutralization (N) 500 µg/mL
Immunohistochemistry (IH) 10 µg/mL
Immunocytochemistry (IC)

10 µg/mL

ELISA 50 ng/mL
Citations and Applications
Nakatsuji T.  Long-term survival of a piece of rat bone marrow graft with the bone in syngeneic rat spleen:  new roles of bone marrow mast cells and host spleen megakaryocytes secreting vascular endothelial growth factor (VEGF).  Haema 2005; 8(3):450-457  Full Text IH, WB

Fairchild Benyo D, Conrad KP.  Expression of the erythropoietin receptor by trophoblast cellsin the human placenta.  Biol Reprod. 1999 Apr;60(4):861-70 Full Text IC

 

Harris KW, Mitchell RA, Winkelmann JC. Ligand binding properties of the human erythropoietin receptor extracellular domain expressed in Escherichia coli. J Biol Chem. 1992 Jul 25;267(21):15205-9 Full text WB, N