Antigen Design Services

antigen design for custom antibody development

Let our team assist you from the start to ensure the success of your custom polyclonal antibody project! There must be many things taken into consideration before starting an antibody program in any species. With over 20 years of experience let one of staff members assist you in developing a plan that is tailored to meet your specific needs.

Antigen Design

Let our highly skilled staff assist you with your antigenic selection and design. When producing polyclonal antibodies it is important to take into consideration a number of factors:

  • What application(s) do you intend to use the antibody for
  • Choosing the best sequence in relation to your target antigen
  • Avoiding epitopes that may be unavailable due to structural constraints
  • Accessing any possible wanted or un-wanted cross reactivity

Antigenic peptide epitopes can be selected using software available at The Immune Epitope Database and Analysis Resource (IEDB) and also lists of ranked peptide epitopes can subsequently searched with BLAST. For more information on these services or to submit an antigen of interest for analysis please contact us at info@capralogics.com or 413.477.6866.

Peptide Synthesis

Capralogics Inc. can assist you in synthesizing your peptide of interest for antibody production. For most antibody production projects, a peptide of at least 85% purity is recommended.  If requested, we can provide a higher purity antigen for an additional charge. Capralogics will synthesize approximately 20mg of peptide, which is generally enough peptide to conjugate for the immunogen, perform ELISA or WB work, and construct a peptide affinity matrix column, if desired.

For 20mg of peptide, the per residue pricing is $36 for peptides up to 25 amino acids in length at >85% purity and includes documentation and HPLC analysis.  For peptides larger than 25 amino acids there is an additional fee of $135 for synthesis.

Peptide acetylation and amidation are offered for an additional fee of $50. Other modifications are available upon request and will be quoted on an individual basis.  Please inquire for prices on lesser or greater peptide amounts, higher purity, and varying residue lengths. Get started today, e-mail your amino acid sequence to info@capralogics.com or fill out our Service Order Form.

Conjugation

To increase the immunogenicity of your antigen you may need to couple it to a larger carrier protein. Generally peptides less than 13-15 KD or less than 25-27 amino acids may need to be conjugated. You can attach your antigen to KLH, BSA or other carrier proteins upon request.

Conjugation of up to 5mg peptide to KLH or BSA using homo or heterobifunctional cross linking agents $300.

Share/Save

IHC Application

Looking to produce and antibody for use in an IHC application? Ask us about producing application specific antibodies.

Capralogics was able to recommend a peptide sequence that produced a high titer antibody and worked well in my intended application of use. Thank you.

-Research Customer