capralogics

capralogics
Sheep anti–Histone H3

DATASHEET


Description: The immunogen prepared for sheep housed at Capralogics was intact calf histone H3 at 15.4 kD, 136 amino acids, complexed with RNA. Calf histone H3 has 100% identity to many other mammalian histone H3 proteins.

Cross-Reactivity: Minimal with linker histone H1 and no cross-reactivity with histone core proteins H2A, H2B, or H4.

Product: Protein G purified immunoglobulin in phosphate buffered saline, pH 7.4. Large volumes of antisera to Histone H3 available for sale.

Storage: Aliquot and store at -20° C or store at 4° C with the addition of 0.1% sodium azide.  Avoid repeated freeze-thaw cycles.

Related Products: Antibodies to histone core, histone H1, and histone H2B

For Research Purposes Only

info@capralogics.com

FAX 1-413-643-0067
Phone 1-800-975-6866

Product Number P00006

Price   buy online
Amount 250 µg
Host Sheep housed at Capralogics
Antigen

Intact calf histone H3-RNA complex

Purification Protein G purified immunoglobulin in phosphate buffered saline, pH 7.4
*
Applications  
Immunoprecipitation (IP) 1:1000
Western Blotting (WB) 1:300
Immunocytochemistry (IC) 1:100
Citations and Appplications
Bradley C, van der Meer R, Roodi N, Yan H, Chandrasekharan MB, Sun ZW, Mernaugh RL, Parl FF. Carcinogen-Induced Histone Alteration in Normal Human Mammary Epithelial Cells. Carcinogenesis. 2007 Oct;28(10):2184-92  Full Text WB, IC
Capozzo AV, Burke DJ, Fox JW, Bergmann IE, La Torre JL, Grigera PR.  Expression of foot and mouth disease virus non-structural polypeptide 3ABC induces histone H3 cleavage in BHK21 cells. Virus Res. 2002 Dec;90(1-2):91-9.  Abstract WB