Capralogics, Inc., P.O. Box 356, Hardwick, MA 01037 USA
Phone (800) 975-6866 / (413) 477-6866 • FAX (413) 643-0067 • capralogics.com

anti-5-methyl cytosine

$200.00
SKU: P00704
Synonyms: 
5-methyl cytosine as 5mC, m5C, 5-Me, and 5-MeC

Immunocytochemistry: Positive staining of rat neuron cells blocked with 2% BSA/1% tween 20 in PBS & incubated @ 4C overnight with an antibody dilution of (5ug/ml)

Specifications

Amount: 
250 ug
Host Species: 
Sheep
Antigen: 
5-methyl Cytosine coupled to coupled to keyhole limpet hemocyanin (KLH)
Format/Conjugates/Purification: 
Protein G
Buffer & Formulation: 
Purified IgG in PBS with no preservatives
Storage: 
Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
Clone: 
Polyclonal

Applications

Western Blotting: 
1:1000
IHC: 
10ug/ml
Immunoprecipitation: 
25-100 ug/ml
References: 

Shirakawa T, Yaman-Deveci R, Tomizawa S, Kamizato Y, Nakajima K, Sone H, Sato Y, Sharif J, Yamashita A, Takada-Horisawa Y, Yoshida S, Ura K, Muto M, Koseki H, Suda T, Ohbo K. An epigenetic switch is crucial for spermatogonia to exit the undifferentiated state toward a Kit-positive identity. Development. 2013 Sep;140(17):3565-76. Abstract IH

Park IY, Sohn BH, Yu E, Suh DJ, Chung YH, Lee JH, Surzycki SJ, Lee YI.   Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein. Gastroenterology. 2007 Apr;132(4):1476-94.   Abstract (Blotting)

Katoh M, Curk T, Xu Q, Zupan B, Kuspa A, Shaulsky G.  Developmentally regulated DNA methylation in Dictyostelium discoideum. Eukaryot Cell. 2006 Jan;5(1):18-25.  Full Text (Blotting)

Park IY, Sohn BH, Choo JH, Joe CO, Seong JK, Lee YI, Chung JH.  Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development. J Cell Biochem. 2005 Feb 15;94(3):585-96. Abstract  (Blotting)

 Fisher O, Siman-Tov R, Ankri S.  Characterization of cytosine methylated regions and 5-cytosine DNA methyltransferase (Ehmeth) in the protozoan parasite Entamoeba histolytica.  Nucleic Acids Res. 2004 Jan 9;32(1):287-97. Full Text  (Affinity Chromatography and Blotting)

Luo S, Preuss D.  Strand-biased DNA methylation associated with centromeric regions in Arabidopsis.  Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):11133-8.  Full Text  (Immunoprecipitation)

Poehland R, Al-Rostum F, Becker F, Viergutz T, Brunner RM, Kanitz W, Bhojwani S.  Donor Cell Lines Considerably Affect the Outcome of Somatic Nuclear Transfer in the Case of Bovines. J Reprod Dev. 2007 Aug;53(4):737-48 Full Text  (Immunocytochemistry)

Mohana Kumar B, Jin HF, Kim JG, Song HJ, Hong Y, Balasubramanian S, Choe SY, Rho GJ.  DNA methylation levels in porcine fetal fibroblasts induced by an inhibitor of methylation, 5-azacytidine. Cell Tissue Res. 2006 Sep;325(3):445-54.  Abstract  (Immunocytochemistry)

 Loukinov DI, et al.  BORIS, a novel male germ-line-specific protein associated with epigenetic reprogramming events, shares the same 11-zinc-finger domain with CTCF, the insulator protein involved in reading imprinting marks in the soma.  Proc Natl Acad Sci U S A. 2002 May 14;99(10):6806-11  Full Text  (Immunohistochemistry)

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Call (800) 975-6866

info@capralogics.com

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